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2 days ago For the first time in history, an indigenous tribe in Cambodia has raised white cranes. The village is now home to three who are the first descendants ofÂ .Biotin and streptavidin, proteins of about Mr=25 000, bind biotin and streptavidin with very high affinity, ca. 10(12) M-1. The conjugates formed between biotin or streptavidin and different small molecule ligands, in particular between biotin and lipophilic vinyl compounds, are among the most stable chemical bonds known. More than 40 such conjugates with a wide variety of natural or synthetically prepared ligands have been prepared. All of the biotin derivatives are extremely potent inhibitors of blood Coenzyme A reductase, a key enzyme in the biosynthetic pathway of Coenzyme A (CoASH), an intermediate in the biosynthesis of fatty acids. The derivatives of streptavidin are useful for detection, purification and immuno-purification of avidin-binding biotinylated ligands.
A limiting factor in many of the known biotin derivatives is the stability of the linkage formed between biotin and the ligand. In the compounds in which biotin is linked through the ester group at the 7-position of its aglycone, this linkage is generally very labile in aqueous environments. The linkage thus often hydrolyses during chemical preparation and purification or during storage of the conjugates. With this linkage, the biotin must be derivatized with a small molecule such as a glyceryl group. Unfortunately, the most labile of these compounds is not very useful since they have significantly reduced biopotency.
In the compounds in which the ligand is attached to a biotin moiety through a chemical bond formed by reaction of two side-chains in biotin (for example, see U.S. Pat. No. 4,447,487), the lability of the linkage is less severe but this has been achieved at the cost of less than optimal biopotency.Q:
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. “The year is 2016 and Robin is now a teenager at high school. In 2016, the young feline had a â€œ.
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. “The year is 2016 and Robin is now a teenager at high school. In 2016, the young feline had a “.
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In the episode, 14, Robin made a wish that if she ever wanted something, it would be granted to her by the last person who had.
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